Journal: PLoS ONE

Article Title: On the influence of cannabinoids on cell morphology and motility of glioblastoma cells

doi: 10.1371/journal.pone.0212037

Figure Lengend Snippet: A) shows the mean speed and standard error of the mean (sem) of single LN229, U87 and U138 cells, when treated with cannabinoid receptor inverse agonists. B) and C) depict the directionality and contact area of the same cell lines and treatments together with the sem. For all parameters cell line specific changes that have no apparent receptor specificity can be observed. Statistics was performed using a Kruskal-Wallis test and significance was chosen for p<0.05. The asterisk denotes significant results regarding the respective measurement indicated with the bar.

Article Snippet: U87 and LN229 cells were purchased from the American Type Culture Collection (Manassas, VA, USA; U87: ATCC HTB-14; LN229: ATCC CRL-2611) and U138 cells were obtained from Cell Lines Service (Cell Lines Service, 300363).

Techniques:

Journal: PLoS ONE

Article Title: On the influence of cannabinoids on cell morphology and motility of glioblastoma cells

doi: 10.1371/journal.pone.0212037

Figure Lengend Snippet: A) shows the mean circularity and sem of single LN229, U87 and U138 cells, when treated with cannabinoid receptor inverse agonists. B) and C) depict the apparent brightness and homogeneity of the same cell lines and treatments together with the sem. For all parameters cell line specific changes that have no apparent receptor specificity can be observed. Statistics was performed using a Kruskal-Wallis test and significance was chosen for p<0.05. The asterisk denotes significant results regarding the respective measurement indicated with the bar.

Article Snippet: U87 and LN229 cells were purchased from the American Type Culture Collection (Manassas, VA, USA; U87: ATCC HTB-14; LN229: ATCC CRL-2611) and U138 cells were obtained from Cell Lines Service (Cell Lines Service, 300363).

Techniques:

Journal: PLoS ONE

Article Title: On the influence of cannabinoids on cell morphology and motility of glioblastoma cells

doi: 10.1371/journal.pone.0212037

Figure Lengend Snippet: A) shows the correlation between apparent brightness and homogeneity of the three cell lines, with an correlation coefficient of r = 0.848 [0.831;0.863]. B), C), D), E) and F) illustrate the correlation between the single cell parameters brightness and contact area, homogeneity and contact area, circularity and contact area, brightness and circularity as well as cell speed and circularity, with correlations coefficients of r = -0.576 [-0.612;-0.536], r = -0.456 [-0.500;-0.410], r = -0.480 [-0.522;-0.435], r = 0.516 [0.473;0.556] and with r = -0.122 [-0.177; 0.066], respectively. Red dots correspond to LN229 cells, blue ones to U138 cells and green dots to U87 cells. The black line corresponds to the respective linear fit.

Article Snippet: U87 and LN229 cells were purchased from the American Type Culture Collection (Manassas, VA, USA; U87: ATCC HTB-14; LN229: ATCC CRL-2611) and U138 cells were obtained from Cell Lines Service (Cell Lines Service, 300363).

Techniques: Single Cell

Journal: PLoS ONE

Article Title: On the influence of cannabinoids on cell morphology and motility of glioblastoma cells

doi: 10.1371/journal.pone.0212037

Figure Lengend Snippet: A) depicts the phalloidin (green) and DAPI (blue) staining of LN229 control cells on the left and the respective structure image of the actin cytoskeleton as a heat map on the right. A correspondence of highly structured regions in the actin staining with the respective structure image is visible. Furthermore, unstructured, homogeneous regions do not contribute to the structure image, as for example in the center of the image. The scaling corresponds to 20 μm. B) shows the quantification of the density of actin structures for U87 cells treated with cannabinoid receptor agonists and inverse agonists as mean value with the sem. C) and D) illustrates the density of actin structures in LN229 and U138 cells after cannabinoid treatment. Various effects that show no apparent receptor specificity were observed. Statistics was performed using a Kruskal-Wallis test and significance was chosen for p<0.05. The asterisk denotes significant results regarding the respective measurement indicated with the bar.

Article Snippet: U87 and LN229 cells were purchased from the American Type Culture Collection (Manassas, VA, USA; U87: ATCC HTB-14; LN229: ATCC CRL-2611) and U138 cells were obtained from Cell Lines Service (Cell Lines Service, 300363).

Techniques: Staining, Control

Journal: Animal Cells and Systems

Article Title: Combined inhibition of STAT and Notch signalling effectively suppresses tumourigenesis by inducing apoptosis and inhibiting proliferation, migration and invasion in glioblastoma cells

doi: 10.1080/19768354.2021.1942983

Figure Lengend Snippet: Activation of Jagged/Notch and STATs in different glioblastoma cell lines and expression correlation of different STATs with Jagged1 in glioblastoma patients. (A) Expression correlation of STAT3, STAT5A and STAT5B with Jagged1 was performed using a TCGA dataset pool of 530 GBM patients. STAT3 ( R = 0.348, P < 0.001), STAT5A ( R = 0.187, P < 0.001) and STAT5B ( R = 0.347, P < 0.001) expression levels significantly correlated with those of Jagged1 in human glioblastoma patients. (B) Western blot analysis of Jagged1, NICD, p-STAT3 and p-STAT5 protein levels in a panel of human glioblastoma cell lines. An astrocyte cell line was used as a normal cell control and GAPDH was used as the loading control.

Article Snippet: Human glioblastoma cell lines (LN18, A172, LN229, and U87MG) and the astrocyte cell line were purchased from the American Type Culture Collection (ATCC).

Techniques: Activation Assay, Expressing, Western Blot, Control

Journal: Animal Cells and Systems

Article Title: Combined inhibition of STAT and Notch signalling effectively suppresses tumourigenesis by inducing apoptosis and inhibiting proliferation, migration and invasion in glioblastoma cells

doi: 10.1080/19768354.2021.1942983

Figure Lengend Snippet: STAT inhibitors induce Notch signalling in glioblastoma cells. A panel of glioblastoma cell lines (LN18, LN18-EGFRvIII, LN229, A172, U87MG-EGFRvIII) were checked for their Jagged1 and NICDs levels by Western blotting 24 h post-treatment in (A) with PMZ (15 µM) and (C) with S3I-201 (100–300 µM). Vehicle only (DMSO) was used as control. GAPDH served as a loading control. Relative cellular mRNA levels for Jagged1, Notch1 and Notch target genes (Hes1, Hey1, Hey2, Hrt2) were quantified by RT-qPCR in LN18 and LN18-EGFRvIII cells treated in (B) with PMZ (15 µM) and (D) with S3I-201 (300 µM) 24 h post-treatment. DMSO only treatment was used as control, with relative expression defined as 1.0. Individual samples in the graphical data are shown with mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, using the Student’s unpaired t -test.

Article Snippet: Human glioblastoma cell lines (LN18, A172, LN229, and U87MG) and the astrocyte cell line were purchased from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Control, Quantitative RT-PCR, Expressing

Journal: BioImpacts : BI

Article Title: Synergism of d-limonene and temozolomide on migratory and apoptotic behaviors of human glioblastoma cell lines

doi: 10.34172/bi.2023.27681

Figure Lengend Snippet: Inhibitory effect of DL+TMZ on U87MG and LN229 cell line

Article Snippet: The U87MG and LN229 human cell lines were obtained from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: